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NERNST probes the NADP(H) redox status in isolated leaf protoplasts Plant protoplasts display features that make them attractive as an experimental model system: they retain important properties of the original cells, can be easily isolated from mutant or transgenic plants, and can be transformed in vitro leading to transient expression of genes of interest

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Following phagocytosis and recognition of MSU crystals in the joints, IL-1 expression is induced by signaling pathways such as TLR, NOD-like receptor 3 (NLRP3), P2X purinoceptor 7 and mitogen-activated protein kinase pathways, among others, leading to an inflammatory response

This paradigm shift operates through: (i) dual-function targeting (simultaneous antithrombotic action and regenerative promotion), (ii) spatiotemporal inflammatory reprogramming (controlled macrophage polarization), and (iii) synergistic regenerative factor delivery (orchestrated release of cardioprotective mediators) [62, 94](Fig

Although later studies have suggested that the timing of HRT initiation may play a critical role, indicating that starting HRT near the onset of menopause might reduce risks, the overall concerns about its safety in postmenopausal women continue to persist [104]