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To make sure that the result was reproducible , I soaked my favorite paintbrush the brush that started this whole experiment- in rubbing alcohol for 2 hours

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Otherwise, bile duct-ligated rats commonly presented intracranial (superior sagittal sinus), portal and caval hypertension and aortal hypotension, gross brain swelling, hemorrhage and lesions, heart dysfunction, lung lesions, liver and kidney failure, gastrointestinal lesions, and severe arterial and venous thrombosis, peripherally and centrally