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After incubation for 10 min, 200 L luciferin solution (containing 20 mM tricine, 1.0 mM MgSO4, 0.1 mM EDTA, 33 mM dithiothreitol, 270 M coenzyme A, 500 M D-luciferin, and 530 M ATP, pH 7.8) was added, and then the firefly luminescence was measured with a BioTek synergy 4 Multi-Mode Microplate Reader

T., Noda, M., O'Regan, A

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Glutamate induced the formation of peroxynitrite, mainly localized within the mitochondria, and triggered delayed (6 h) calcium overload, mitochondrial depolarization, lipid peroxidation, and massive neuronal cell death

Fluxomics with ratiometric metabolite dyes

115 These mutations lead to spontaneous inflammasome formation and IL-1, IL-18 production together with cell pyroptosis in the absence of the stress signal