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Celiac disease or Crohn's disease Alcohol use disorder (AUD) Malabsorption syndrome Older age (75+ years) Dietary patterns lacking in vitamin B-rich foods, such as animal products (meat, eggs, dairy foods) History of gastrointestinal surgery, including gastric bypass surgery for weight management Use of certain medications that impact the body's utilisation of specific B vitamins Individuals at higher risk of encountering reduced levels of vitamin B12 include older adults, children, individuals following vegan or vegetarian diets, individuals with diabetes, those who have undergone gastric bypass surgery, breastfeeding women, and individuals with conditions affecting absorption like Crohns disease

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[The Working Group noted that a unique feature of this cohort was that its large size allowed restriction to never-smokers and stratification by sex

The lysate was centrifuged at 7000 g for 20 min to remove cell debris and unbroken cells, and the supernatant was subjected to ultracentrifugation at 200,000 g for 30 min to pellet the cell membranes

doi: 10.1161/01.ATV.0000201938.78044.75 156 MohammadHMFGalal GoudaSEladlMAElkazazAYElbayoumiKSFaragNEet al