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Description

Prostate 28 , 251265 (1996)

10.1136/annrheumdis-2020-217424 86

The GST-Rev1 fusion protein was purified from bacterial lysate by affinity chromatography using a glutathione-Sepharose column

After completion of the NCL ligation (2 h), the second step of the process that finalizes the assembly of the linear peptide K1 was performed by adding the SEA peptide K1 [ 125148]-SEA 12 and TCEP to the reaction mixture