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d ) lyso-H 4 B-CH 3 (10 nM) and lysoTracker DR (75 nM) to show lysosomal localization of lyso-H 4 B-PMHC

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To further elucidate the catalytic mechanism of how P300-mediated H3K18la, we performed molecular docking analysis using Autodock Vina to investigate the interaction between P300 and lactyl-CoA (PubChem: 1926-57-4), aiming to determine their binding mode and active sites (Fig

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Experiments were executed at 25C in the early afternoon

Subsequently, absorbance at 450 nm was measured using a microplate reader (Molecular Devices, CA, United States)