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LHCGR engagement activates Gs-coupled adenylyl cyclase, driving cAMP accumulation and PKA-mediated phosphorylation of downstream steroidogenic targets including CYP11A1, CYP17A1, and 3-HSD

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The participants continued to apply 4 sprays (0.5 mL or 100 mg rGSH) of the topical solution twice a day (6 h apart) until the final blood draw 72 h after the initial application