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Choong CK, Haddad FJ, Martinez C, Hu DZ, Pierce J, Meyers BF, Patterson GA, Cooper JD: A simple, reproducible, and inexpensive technique in the preparation of explanted emphysematous lungs for ex vivo studies

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As conservative therapy and percutaneous procedures are quite palliative in nature and may restrict functionality, surgery is utilized to help alleviate discogenic pain and with the hope of providing better mobility and function

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Thus, in our in vitro model of DR, ARPE-19 cells were cultured in DMEM/F12 nutrient mixture having the recommended standard glucose concentration of 17.5 mM, as control condition