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The formation and identification of metabolites over time were analyzed using HPLC-DAD and LCMS n techniques

For H 2 O 2 assessment in whole cell, 1.510 4 cells were incubated in 100 M AmplexRed in Krebs-Ringer Phosphate buffer (145 mM NaCl, 5.7 mM Na 2 PO 4 , 4.86 mM KCl, 0.54 mM CaCl 2 , 1.22 mM MgSO 4 and 5.5 mM glucose, pH 7.4), and the luminescence recorded as we did for mitochondria

switching d4T to TDF, ABC, 3TC or FTC, or eliminating PI when safe)

2020;6(35):eabb7878

Sample preparation Calibration curve for 2 was prepared by spiking 40 l of blank mouse plasma with 10 l of working standard solution to get final concentration ranging from 2.5 ng/ml 2000 ng/ml

Immune system: Vitamin D3 helps regulate the immune system, so it responds properly to pathogens