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The supernatants were added to 96-well plate and adjusted to the volume of 100 l with assay buffer

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Several signs and symptoms should alert you to a possible vitamin B deficiency: digestive disorders (diarrhoea, constipation), skin issues (rashes, cracks around the mouth, for example), tiredness, anaemia and occasionally neuropsychiatric disorders (irritability, depression)

10.3390/ijms23137109 162

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Specifically, we are proposing to make certain technical and clarifying changes to the information that must be submitted for an expansion exception request