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Briefly, Salmon v1.9.0 77 and the fq v0.9.1 ( were used to sub-sample FastQ files and auto-infer read strandedness

The membranes were washed 3 times with 1xTBS containing 0.05% Tween and incubated with a 1:10000 dilution of HRP-linked goat anti-rabbit IgG polyclonal antibody (Cell signaling Technology, Danvers, MA, USA)

In recent decades, natural products with diverse bioactive properties have gained attention as alternative strategies for combating various cancer types

Together, they are commonly studied for their combined influence on appetite-related pathways, energy intake signaling, and metabolic regulation markers