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For ROS detection, peptide conjugate solution (400 M) and 10 M DCFH were mixed in a 1:1 (v/v) ratio, yielding a final peptide concentration of 200 M in a total volume of 100 l per well in 96-well plate

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doi:10.1051/medsci/2007233297

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ImageStream analysis Cells were loaded into an Amnis ImageStreamX Mark II system (EMD Millipore) for imaging and fluorescence detection

By weaving together these strands, we aim to provide oral and maxillofacial surgeons with a metabolic framework that informs perioperative nutrition, guides future clinical trials, and ultimately enhances patient recovery in the rapidly growing geriatric population