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National Institutes of Health Office of Dietary Supplements

Statistical analysis All statistical analyses were done using the SPSS software (version 23

The supernatant containing polar metabolites was evaporated to dryness by SpeedVac

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After one month of their habitual diet, which was designed to reflect serum L-Cn fluctuation, the volunteers were shifted to a weeklong beef-rich diet (around 220 g cooked beef during each lunch and dinner, equivalent to an estimated 330 mg L-Cn per day)