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However, in the TME, which is characterized by hypoxia and high levels of immunosuppressive metabolites, the function of NK cells is often severely suppressed by high levels of ROS

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After washing with PBS, the nuclei were counterstained with Hoechst 33342 (1:1000), and coverslips were mounted with Dako fluorescent mounting medium (S302380-2, Agilent Technologies)

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