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Step one: baseline documentation Before starting any treatment, document your current status

Birchmeier C, Birchmeier W, Gherardi E and Vande Woude G F (2003) MET, METASTASIS, MOTILITY AND MORE, in Nature Reviews Molecular Cell Biology pp 915-925, Nature Publishing Group

The resultant PCR products of N1, N2, and F0362 were cloned into either pQE80L or pQE30 expression vectors (Qiagen, Valencia, CA), which encodes a 6xHis-tag at the N-terminus

Data were acquired with a QExactive HF-X hybrid quadrupole-orbitrap mass spectrometer coupled to a Thermo Scientific Vanquish Flex Ultra-High Performance Liquid Chromatography system (Bremen, Germany)

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Inject on an empty stomach (avoid food 23 hours before and 3060 minutes after) to optimize GH release