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Briefly, GSTO1-1 pQE30 plasmid was transformed into M15 cells, which were grown in Luria-Bertani broth supplemented with 100 g ml 1 ampicillin and 25 g ml 1 kanamycin at 37 C to an OD 600 of 0.8 and induced with 0.1 mM isopropyl thio- -D-galactoside for 4 h

The total run time per sample was 20 min

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Identification of a tumor-promoter cholesterol metabolite in human breast cancers acting through the glucocorticoid receptor

People with low kidney function may need to limit the amount of protein in their diets (5)

The results showed that the total score of ABC was significantly negatively correlated with R, cortisol, taurine, methionine, histamine, and homocysteine, and positively correlated with creatinine