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Mechanistically, microbial dysbiosis exacerbates renal injury and fibrosis by increasing intestinal permeability, disrupting lipid metabolism, inducing ROS-mediated oxidative stress, and activating TLR4-mediated inflammatory responses ( Figure 4 ) [128]

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2.13 Immunoblot detection of heme oxygenase 1 Heme-dependent heme-oxygenase 1 (HMOX1) induction was used to provide evidence of heme delivery by HPX to HepG2 and primary liver hepatocytes as described in the Supplementary Material