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Studies in mice revealed that dietary supplementation with TMAO, choline, or carnitine decreased reverse cholesterol transport (RCT) (Janeiro et al., 2018), a mechanism that counteracts excess cholesterol deposition in peripheral tissues by transporting excess cholesterol to the liver and small intestine (Ross and Glomset, 1973).The major pathway for cholesterol elimination is the metabolic synthesis of bile acids in the liver (Zarate et al., 2016), and it was reported that the size of the total bile acid pool was significantly smaller in mice administered TMAO (Koeth et al., 2013)

As the UV spectra of both compounds are very similar and the ECD spectra exhibit more differences, it is probable that the different substituents affect preferable conformations, which the two compounds adopt in aqueous solutions