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The Lp02 dupA dupB mutant was described earlier 9

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Mater Design 47:424442 Bates LS, Waldren RP, Teare I (1973) Rapid determination of free proline for water-stress studies

The cells were then harvested, centrifuged, washed twice with PBS, resuspended in a volume of PBS (500 L), and analyzed using flow cytometry

(More information) The developing brain may be particularly vulnerable to deficiencies in choline and essential fatty acids

Precise understanding of immunometabolic networks has been limited due to low abundance of DC subsets in the blood as well as challenges associated with bulk metabolic measurement which may not reflect natural temporal stochasticity and more recently recognized heterogeneity of ex-vivo cell cultures 22,23