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They have a diverse microbial community in their rumen which includes bacteria capable of synthesizing vitamin B12

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Using anything less reliable often leads to contamination, reduced potency, or inconsistent data, issues that can compromise entire experiments

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Nevertheless, various studies reported that an extensive pre-purification (Toll et al., 2006) or specific solid-phase extraction (Goumenou et al., 2025) yielded a mass spectrum of the intact subunit