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Samples were separated by SDS-PAGE (NuPage 412% Bis-Tris Gel, Life Technologies), run with BioRad Precision Plus Protein Kaleidoscope ladder, and transferred to a nitrocellulose membrane using an iBlot system (Life Technologies)

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SIRT6, an NAD + -dependent histone deacetylase, has been shown to be downregulated in both mouse models of ALD mice and patients with AH

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Huynh et al., 2015) and 65 to 90 percent of patients with advanced cirrhosis (Henkel and Buchman, 2006)