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Description

We next performed a structural similarity search across our in-house compound collection, as well as on commercial small-molecule libraries (1,000,000 compounds) and identified 141 additional hits, which were screened in the high-throughput activity assay at a single concentration of 10 M

Lysates were transferred into protein low binding tubes and sonicated for 10 cycles (30 seconds ON, 30 seconds OFF) at 4 C with the Bioruptor Pico instrument (Diagenode)

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