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Following fixation, L3 filets were washed in PTX (PBS and 0.02% Triton X-100) and blocked in PBT (PBS, 0.02% Triton X-100 and 1% NGS) on a nutator and incubated with primary antibodies for either 2 h at room temperature or overnight at 4 C with agitation

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This is especially challenging scenario for a patient such as myself since I rely on the signature of the medical professionals/psychiatrists to obtain my subsidized health insurance from the US Government and my subsidized weekly food basket from the Church (I have been poor for many years because my neurological symptoms were so severe than I have had to rely on government subsidized health insurance and charity food baskets from the Church in order to survive because I was repeatedly fired from my jobs due to neurological problems.) The situation of relying on the psychiatrist signature for food, forces the patient into lying to survive and then further increases the attitude/bias against those with Bipolar/Schizophrenia by further labeling them with Borderline Personality Disorder, a diagnosis associated with the behavior of being a manipulative liar, further bias confirmed when the patient fails the urine test used to detect the levels of prescribed Lamotrigine/Antipsychotics for Compliance

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