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Activation of SSAT1/ALOX15 axis aggravates cerebral ischemia/reperfusion injury via triggering neuronal ferroptosis

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Trypan blue for cell death and DAB staining for H 2 O 2 accumulation Detached leaves were immersed in trypan blue staining solution (2.5 mg ml 1 trypan blue dissolved in lactophenol:lactic acid:glycerol:liquid phenol:ddH 2 O at 1:1:1:1:1) for 30 min

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41 When T cells are deprived of Trp and Arg, activated T cells cannot enter the S phase, which proves that Trp and Arg are key substances for T cells to enter the cell cycle