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The samples were then incubated overnight at 4 C with primary antibodies -III-tubulin (1:100, ab7751, clone TU-20, lot GR3238448-11, Abcam), -SMA (1:25, ab7817, clone 1A4, lot GR119216-7, Abcam), type III collagen (1:100, Acris AF5810, clone III-53, lot A130097BH) and CD45 (1:100, ab23, clone UCH-L1, lot GR3189280-2, Abcam) in 2.5% BSA