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For protection assays, crude venom was preincubated (30 min at 37 C) with varying cytotoxin:binder ratios of 1:1, 1:2.5 and 1:5 before injection ( n = 3) (ratios estimated from the proportion of cytotoxin in the venom)

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The examiner inspects the cigars for imperfections and checks them for proper weight, size, shape, and condition of the wrapper

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