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High doses of B12 may worsen the disease and cause irreversible damage to the optic nerve

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Alcohol Clin Exp Res 2004;28:131136

The cells were then permeabilized and blocked using 0.5% Triton X-100 and 5% BSA, respectively and then incubated with anti-NOX2 primary antibody (1:500, Proteintech) at 4 C overnight and with fluorescence dye-conjugated secondary antibodies at 37 C 1 h