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Participants need not worry about being prosecuted, as it does not presume they are using or have used vaping products

When data were below level of detection, the most conservative approach was adopted by taking the middle value of the range between the limit of quantification and detection for analysis (LD + (LQ-LD)/2)

However, once tobacco is added, whether in a blunt or a spliff, the risks increase significantly

In 2017, cigarette companies spent $9.36 billion on marketing cigarettes and smokeless tobacco in the U.S., based on data from the Federal Trade Commission (FTC).[42] The amount converts to $25 million a day, or over $1 million per hour

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Similarly, in vivo and in vitro data also support that acute exposure to aerosolized PG:VG increases Mucin-5AC (MUC5AC) protein levels [34], a major airway gel forming protein known for its role in preventing infections [35] and a biomarker for COPD severity [36]