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7 (11) e11139

This is the knockout punch

Tong Y, Zhang J, Sun N, et al

The analysis of allele frequencies in candidate gene variants and SNPs holds significant implications for various scientific domains, including uncovering the genetic correlations with diseases and health-related traits, and estimating the prevalence of individuals predisposed to certain conditions or drug resistance within populations [47]

Next, a Bertin 24 homogenizer (Bertin Technologies) was run at 5,000 rpm (2 cycles of 45 s with a pause of 5 s in between) to lyse the microbial cells