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The redox potential of the glutathione system is better expressed as the redox state of the GSSG/2GSH redox couple, calculated from the Nernst equation, which takes into account the reducing capacity of the redox couple, with the stoichiometry of two GSH molecules per GSSG molecule (Schafer & Buettner, 2001)

Messerer, J

At proper refrigeration temperatures, the water molecules move slowly enough that they rarely have enough energy to break peptide bonds

Briefly, a 0.4 mM DPPH solution in methanol was vigorously mixed with various concentrations (5-100 mg/ml in 60% ethanol) of CME

Here, a simple and efficient assay was established which is based on the quantification of the second reaction product of carnitine monooxygenase (Figures 3A,B)

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