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2.6 Quantitative real-time polymerase chain reaction (qRT-PCR) Total RNA was extracted from PFC and NAc samples using Direct-zol RNA MiniPrep Kit (ZYMO-RESEARCH, United States

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This percentage increased to 36.2% when asked about the harmful effects of vaping and smoking waterpipes

"This means that they generally contain fewer harmful ingredients compared to traditional tobacco products," he said

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