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Mitophagy is crucial for sustaining healthy mitochondria and serves a protective function by eliminating damaged mitochondria and protein aggregates (Liang and Kobayashi, 2016

3,3'-diaminobenzidine (Vector Laboratories) was used as the peroxidase substrate

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Then, cells were placed in a selective medium containing hygromycin B (100 g/mL for MCF-7 and 200 g/mL for SUM-159 and MDA-MB-231)