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In the pathological state, O 2 reacts with NO to increase ONOO via an up-regulation of NOS and endothelial NOS [50]

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After incubation, mitochondria were pelleted (1 min at 16,000 g ) and pellets were resuspended in 200 L of 50 mM HEPES buffer (pH 7.4) supplemented with 10 mM IAM-TPP, disrupted using mechanical micro-homogeniser and sonicated for 5 min

Semaglutide and walking capacity in people with symptomatic peripheral artery disease and type 2 diabetes (STRIDE): a phase 3b, double-blind, randomised, placebo-controlled trial

Proc Natl Acad Sci USA 96: 46154620

doi: 10.1038/s41586-024-08146-w 120 Samboln-EscobalesL.Tirado-CastroL.SuarezC.Pacheco-CruzD.Fonseca-FerrerW.DemeP.et al