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Then, 100 L homogenate was transferred to a labeled 1.5 mL centrifuge tube, 300 L methanol added, and the contents mixed on a vortex mixer for 1 h

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Patients typically use the thigh, abdomen, or upper arm as injection sites

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Even small amounts of selenium are sufficient for correct DIO activity, but a reduced selenium intake affects GPx expression and function, leading to impaired antioxidant activity in thyroid cells

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