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Gene Expression Profiling To broaden the understanding of the effects of these lysosomotropic compounds on the cells, we selected four compounds to conduct gene array profiling analysis

However, these methodologies are often multistep procedures and present different shortcomings such as non-specific adsorption, leakage, and/or partial denaturation of immobilized biomolecules [616,617]

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However, our previous study revealed that fusion-expressed multivalent nanobodies exhibited reduced affinity, possibly due to the spatial structure blocking the binding epitope 13