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A section on computational modelling algorithms using information from crystal structures and ligands is included to inform readers about the use of in silico methods to gain information about transportersubstrate interactions

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The challenge with many interventions is that they lack selectivity, affecting multiple physiological systems simultaneously and creating side effects that limit their practical application or raise safety concerns

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Mutation of E 74 to glutamine (Q) also induced helical formation in the CHAC2 loop 2, suggesting it might be the catalytically relevant glutamate residue that alternates with E 83 (Nguyen et al., 2020)