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After an overnight incubation at 4 C with primary antibodies against FAT/CD36 and CPT (Santa Cruz Biotechnology, USA), the membranes were washed with PBS-T and incubated with an HRP-conjugated secondary antibody for 1 h at 25 C

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However, it seems that long-term L-carnitine intake eventually converts to a metabolite called trimethylamine N-oxide (TMAO) (9799), and increased circulating levels of this metabolite can increase the prevalence of diabetes (54% per 5 mol/L increment of plasma TMAO) (100), nonetheless one study showed that increased TMAO plasma level by 30 mol/L after 24 weeks of L-carnitine supplementation was not related to glucose, insulin and HOMA-IR levels changes (97)