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Biogerontology 20 , 381395 (2019)

ENNs are cytotoxic through the disruption of normal physiological concentrations of Ca 2+ , Na + , K + across membranes by disrupting ionic selectivity, which is debilitating for mitochondrial membranes causing uncoupling of oxidative phosphorylation [320,321]

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coli Rosetta2DE3pLysS cells transformed with recombinant plasmids were grown to an A 600 of 1.0 at 30 C and the protein induction was carried out at 18 C for 12 h using 1 mM isopropyl--D-thiogalactoside (IPTG) (MP Biomedicals, 11IPTG0001)

The study was conducted in three phases: (i) participant screening and selection, (ii) dietary intervention, and (iii) post-intervention metabolomic analysis