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During aging, the GSH system in general and the GPx family in particular undergo modifications that promote the production of oxidative stress resulting in disturbances in metabolic regulation, damage of cellular constituents accompanied by cardiovascular follow-up, neurodegeneration, and cancers

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Stem cell therapies promote tissue regeneration through mechanisms including angiogenesis and cellular proliferation

Unless indicated otherwise, cystine-free medium was prepared by supplementing cystine-, methionine- and glutamine-free DMEM (Gibco, CA, USA) with 100 M methionine, 4 mM glutamine, 1 mM sodium pyruvate, 0.2% bovine serum albumin (BSA), 100 U/ml penicillin and 100 g/ml streptomycin

Extracts were clarified by centrifugation and filtration through a 0.45 m pore Spin-X filter (Corning), and the total protein concentration was determined by BCA assay